A Comprehensive Review of Fluorescence Correlation Spectroscopy

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

This article focuses on methods based on fluctuation correlation spectroscopy to determine the formation of protein complexes in living cells. We present the principles of the fluctuation method applied to cells. We discuss the novelty and the promises of this approach. The emphasis is in the discussion of the underlying statistical assumptions of the image correlation spectroscopy analysis rat...

Biological and chemical applications of fluorescence correlation spectroscopy: a review.

The mathematical concept of fluorescence correlation spectroscopy (FCS)1 (1) emerged from quasi-elastic light scattering (QELS) spectroscopy (2) in the early 1970s. Compared to light scattering, the enhanced sensitivity of fluorescence to changes in molecular structure, chemistry, and local environment makes FCS a superior analytical tool for chemical kinetics studies (1, 3-5). The primary moti...

Fluorescence correlation spectroscopy.

Multiphoton Fluorescence Correlation Spectroscopy (MPFCS)

Nanoparticles have been demonstrated as a promising new generation of specific imaging agents and targeted delivery vehicles for the diagnosis and treatment of solid tumors. Nonetheless, for optimal therapy of tumors, the nanoparticles must penetrate the tumor interstitial matrix to reach the cancer cells. This thesis describes the development of a multiphoton fluorescence correlation spectrosc...

Multi-confocal fluorescence correlation spectroscopy.

We report a multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique that combines a Spatial Light Modulator (SLM), with an Electron Multiplying-CCD camera (EM-CCD). The SLM is used to produce a series of laser spots, while the pixels of the EM-CCD play the roles of virtual pinholes. The phase map addressed to the SLM, calculated by using the spherical wave approximation, makes it ...